Need advice

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Mon Apr 9 22:04:34 EST 2001


I am trying to get two ~50bp inserts, each with a sticky and a blunt end to
ligate to a vector (sticky). No luck ... 

Any suggestion regarding conditions, etc.? 

This is the real situation:

Insert A:  P~EcoRI ----  blunt~P
Insert B:  P~BamHI ----  blunt~P

Vector:   ---- EcoRI~OH       OH~BamHI ----

Thanx in advance
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu

---




More information about the Methods mailing list