error rate of polymeases

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Mon Apr 9 22:33:00 EST 2001


Hi all, 

To my knowledge, the error rate of Taq is said to be 1/1000 , 
that of proofreading Pols (Pwo, Pfu) ist said to be 1/5000.

Are these figures close to reality and what do they actually 
mean? When I copy a gene with Taq, 
there will be (statistically) 1 error in every 1000 bases? 
Meaning that after a 30 cycle (theoretical) PCR starting from a 
single copy, there should be 30 errors within 1000 bp (after 
30 duplications)?

Wolfgang
[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
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