Stability of sticky ends.
J. Arturo García-Horsman
arturo.garcia-horsman at uku.fi
Tue Apr 10 00:36:13 EST 2001
> There is someone in my lab who likes to restriction digest a lot of DNA and
> save it in the freezer. I believe that the sticky ends are not completely
> stable, and tend to go away over time. However, I can't remember where I
> learned that so I don't know if it is true or just my superstition.
> Does anyone have any information on the stability of sticky ends?
I have a set of vectors digested with several different enzymes, purified, and
stored in the freezer. Some of them have been there for more than a year or
couple of years. I use them for rutine ligation and I have not noticed any
variation. I don't see any problem with the stability of sticky ends if your
preparation is free of contamination, as in any case!!
Arturo Garcia-Horsman, Ph.D.
Department of Pharmacology and Toxicology
University of Kuopio
Canthia, Harjulantie 1 A, 1. krs.
70210 Kuopio, Finland
E-mail: Arturo.Garcia-Horsman at uku.fi
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