Stability of sticky ends.

J. Arturo García-Horsman arturo.garcia-horsman at uku.fi
Tue Apr 10 00:36:13 EST 2001



CHMC wrote:

> There is someone in my lab who likes to restriction digest a lot of DNA and
> save it in the freezer.  I believe that the sticky ends are not completely
> stable, and tend to go away over time.  However, I can't remember where I
> learned that so I don't know if it is true or just my superstition.
>
> Does anyone have any information on the stability of sticky ends?
>
> Thanks,
>
> Adam

I have a set of vectors digested with several different enzymes, purified, and
stored in the freezer. Some of them have been there for more than a year or
couple of years. I use them for rutine ligation and I have not noticed any
variation. I don't see any problem with the stability of sticky ends if your
preparation is free of contamination, as in any case!!

Arturo

_______________________________________________________________

Arturo Garcia-Horsman, Ph.D.
Research Specialist
Department of Pharmacology and Toxicology
University of Kuopio
Canthia, Harjulantie 1 A, 1. krs.
70210 Kuopio, Finland
Tel: +358-017-162422
Fax: +358-017-162424
E-mail: Arturo.Garcia-Horsman at uku.fi





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