Fishing DNA with Dynabeads !!
srohrer.nospam at immv.unizh.ch
Tue Apr 10 02:19:31 EST 2001
or do the same using biotinylated oligos.
Dima Klenchin wrote:
> Here is what I can think of to achieve such specific
> purification, but it is a lot of work:
> Make uneven synthetic linker that would ligate
> perfectly to your digested product, anneal it
> in vitro, add to your digest, ligate, capture
> to beads carrying an oligo complementary to an
> overhang part of your linker, wash, elute by
> digestion with the same enzyme.
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