DNA sequencing problems
f.jovelin at eurosequence.com
Wed Apr 11 02:10:31 EST 2001
you should have a look here :
Web : http://www.eurosequence.com
E-mail : f.jovelin at eurosequence.com
"Dr. N.A. Salazar" <nsalazar at hgmp.mrc.ac.uk> a écrit dans le message news:
Pine.GSO.4.21.0104092208130.23418-100000 at tin...
> HI There,
> I am having problems reading my DNA sequences and have tried different
> amounts of template and different preparations, but still I get many
> "N"s in the output file. How can I avoid those "Ns" and what are the cause
> of them ?? Where can I read about "troubleshooting" for DNA sequencing ?
> OI am using the ABI System 3700, with the Big Dye Terminator, from
> Applied Biosystems. I alre went to their Website but did not finf any help
> in there.
> I appreciate you comments,
> Nelson Salazar
> nsalazar at hgmp.mrc.ac.uk
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