Mutation detection in DNA fragments

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Thu Apr 12 02:03:41 EST 2001


Costs aside (happy who really can ignore them...) ist also depends a lot
whether you are looking for a specific, known and frequent mutation. Then
Real Time PCR methods or single nucleotide extensions would be good. If you
have to look at whole stretches of genes then you'd rather go for a
screening with SSCP or EMC (enzyme mismatch cleavage) or others and then
confirm by sequencing.

Cheers

Bruno



"Jurgen Sasse" <microbio at emirates.net.ae> wrote in message
news:DEEEKFCOCJDIKOJFHABAEEILCAAA.microbio at emirates.net.ae...
> What is in your opinion the best method for mutation detection in DNA
> fragments of PCR amplifiable size, excluding sequencing in the first
> instance.
>
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