Sv: Sv: Real-time from Corbett-Research

salanti ali at image.dk
Fri Apr 13 02:15:31 EST 2001


Hello again.
I have another question, I know it has been answered before, but I couldn't
find it! Do you have a recipe (or a link) for home brew sybr green buffers
to be used in a real time PCR!
thank you
bdrg Salanti


Dr. Duncan Clark <news@[127.0.0.1]> skrev i en
nyhedsmeddelelse:HiDP9QACVt06EAy+ at genesys.demon.co.uk...
> In article <9aseji$1rse$1 at news.net.uni-c.dk>, the eminent salanti at
> UNI-C wrote
> >So my questions have been answered!
>
> What I would also add, if I haven't already, is that if you are trying
> to totally optimise assays then the SmartCycler is very very useful as
> you can run up to 16 different cycling conditions all at once. For
> higher throughput I would use the RotorGene.
>
> Duncan
> --
> Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR:
>
> Optimisation, Probe Technology & Future Systems
>
> 4-5 September 2001
> King Alfred's College, Winchester, UK
>
> http://www.dera.gov.uk/html/news/events/real_time_quantitative_pcr.htm





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