how to determine the number of plates required for

feixie feixie3 at sina.com
Fri Apr 13 10:29:47 EST 2001


Dear colleagues,
I plan to screen a cDNA library of human testis by homologous
hybridization.
The protein to identify is estimated at 5% of the total protein in human
testis. The library has 10(8) clones/ml, and we could screen up to 10(4)
clones in a 90mm plate. Could you tell me how to determine the number of 
plates required for screening?



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