Home brew Sybrgreen mix for QPCR

salanti ali at image.dk
Sun Apr 15 07:06:03 EST 2001


Hi.
Does anyone have a recipe for Sybr green mix (i.e buffers and quantities)
for QPCR. I would also like to know if anyone has any comments on homemade
Sybr green PCR mixes. Several companies has stressed that it is very
important to use the ready-made Sybr green pcr mixes for QPCR, and extremely
suboptimal to make it yourselv!
Thanks
A.Salanti





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