klenow and ligations

Roee Atlas roee.atlas at weizmann.ac.il
Mon Apr 16 05:22:13 EST 2001


Hi,
 
I'm doing ligation of blunt DNA. To blunt the DNA I'm  using a Klenow
fragment. Is it possible to klenow the DNA fragment after gel excision, then
heat-inactivate the Klenow enzyme and put the blunted fragment straight to
ligation? Do the dNTP's in the reaction disrupt the ligase?

Thanks,
Roy.

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