medium scale production of mammalian proteins
lindow at molpharm.dk
Mon Apr 23 19:39:05 EST 2001
I need 50-100 mg of a protein we have designed. A fusion between a 70
residue protein and the Fc-part of IgG1. We have succesfully expressed
small amounts in cos7-cells and purified on Protein A. It is glycosylated on
the antibody part.
Does anyone have suggestions to a cheap and quick way to make a LOT of it?
We also have a stable HEK293 cell line producing it. Is there any way to
grow such cells at a very density without having to invest in expensive
Alternatively: Is there any commercial custom protein production companies?
All input is welcome.
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