medium scale production of mammalian proteins

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Tue Apr 24 01:05:01 EST 2001


if you have to stick to mammalian cells, you might try stably expressing CHO
cells. They can be adapted to growth in suspension, increasing the yield/ml

bruno

"Dima Klenchin" <klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu> wrote in
message news:9c2dks$1gk_002 at doit.wisc.edu...
> In article <9c1i51$cl6$1 at news.net.uni-c.dk>, "Morten Lindow"
<lindow at molpharm.dk> wrote:
> :Hi group,
> :
> :I need 50-100 mg of a protein we have designed. A fusion between a 70
> :residue protein and the Fc-part of IgG1.  We have succesfully expressed
> :small amounts in cos7-cells and purified on Protein A. It is glycosylated
on
> :the antibody part.
> :
> :Does anyone have suggestions to a cheap and quick way to make a LOT of
it?
>
> Baculovirus in insect cells.
> Yields of ~ 20 mg/l culture are not uncommon
> (provided the expression conditions were carefully
> optimized). That's what I am getting for a protein
> of 145 kDa.
>
> For comparison, Cos-1 expression takes lots of DNA
> and transfection effort and yields about 0.5 mg/l.
>
>         - Dima





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