Inactivation of DNase I

Stefi~ xxxgifalone at tin.it
Tue Apr 24 06:18:58 EST 2001


chopstixn <chopstixn at aol.com> wrote in message
20010424000604.12896.00000674 at ng-cs1.aol.com...
> After DNase treatment on my RNA sample isolated with TRIZOL or similar
> protocol, I usually use phenol or TRIZOL extraction straight away to kill
any
> of remaining enzymes.  It is an additional step, but works and usually the
loss
> of RNA is 10% or less (according to 260nm absorbance).

I abandoned that method because phenol and CHCl3 are hazardous and because
we work with a few RNA so we'd like to lose the least possible.
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911





More information about the Methods mailing list