E. coli cell free lysate

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Tue Apr 24 21:44:39 EST 2001


Hi all, 

I'd like to prepare a cell free lysate from E. coli for a 
qualitative in vitro DNA replication experiment.  Is there some 
easy procedure to try out (preferably lysing with triton 
and some chelators like EDTA or something alike)?

Wolfgang

[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
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