bleach and used LB

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Thu Apr 26 21:13:50 EST 2001


Emir, it's not the chlorine alone, I know that smell and I 
didn't use chromic acid (hypochlorite will destroy even 
plasmids as fa as I know)
I really thought of some halogenation products.
Of course it could be the mixture of chlorine and LB smell and 
some perfume effect.

Wo

> I guess, reduction of hypochlorite results in formation of chlorine gas. You
> probably noticed a brownish color as well. ...Interesting, I also associate
> smell of chlorine with chemical lab. Why? Oh, maybe because of the chromerge
> used to wash the glass!
> :-)
> Emir
> 
> 
> ""Wolfgang Schechinger"" <wolfsc at ibms.sinica.edu.tw> wrote in message
> news:200104261604.f3QG4H825895 at ibms.sinica.edu.tw...
> > Hi all,
> >
> > just sterilized some used LB with bleach (mostly alkaline
> > hypochlorit)  after harvesting the bucks in order to kill the
> > leftovers. after a few minutes i noticed an aromatic smell
> > emerging from the flasks reminding me of my past organic
> > practicals.
> >
> > any ideas what's happening - some nucleophilic halogenation?
> >
> > wolfgang
> >
> >
> > [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> > -----
> > Dr. Wolfgang Schechinger
> > Lab N233 (c/o Dr. Steve Roffler)
> > Institute of Biomedical Sciences
> > Academia Sinica
> > 128 Yen-Chio Yuan Rd. Sec.2
> > Taipei 115
> > Taiwan R.O.C.
> > Tel +886-2-2789-9152
> > Fax +886-2-2782-9142
> > Mobile +886-925-136893
> > wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
> > -----
> >
> > ---
> 
> 
> 
> 
[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
-----
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
-----

---




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