bleach and used LB

a.krimer at biosidus.com.ar a.krimer at biosidus.com.ar
Fri Apr 27 09:11:17 EST 2001


It was phosgene POCl3 (or similar)

Alejandro




Michael Witty <mw132 at mole.bio.cam.ac.uk>@hgmp.mrc.ac.uk on 04/27/2001
06:23:58 AM

Sent by:  owner-methods at hgmp.mrc.ac.uk


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Subject:  Re: bleach and used LB


Just out of interest, how does this chlorine relate to the "gas" or World
War I?  Mike.

On 27 Apr 2001, Wolfgang Schechinger wrote:

> Emir, it's not the chlorine alone, I know that smell and I
> didn't use chromic acid (hypochlorite will destroy even
> plasmids as fa as I know)
> I really thought of some halogenation products.
> Of course it could be the mixture of chlorine and LB smell and
> some perfume effect.
>
> Wo
>
> > I guess, reduction of hypochlorite results in formation of chlorine
gas. You
> > probably noticed a brownish color as well. ...Interesting, I also
associate
> > smell of chlorine with chemical lab. Why? Oh, maybe because of the
chromerge
> > used to wash the glass!
> > :-)
> > Emir
> >
> >
> > ""Wolfgang Schechinger"" <wolfsc at ibms.sinica.edu.tw> wrote in message
> > news:200104261604.f3QG4H825895 at ibms.sinica.edu.tw...
> > > Hi all,
> > >
> > > just sterilized some used LB with bleach (mostly alkaline
> > > hypochlorit)  after harvesting the bucks in order to kill the
> > > leftovers. after a few minutes i noticed an aromatic smell
> > > emerging from the flasks reminding me of my past organic
> > > practicals.
> > >
> > > any ideas what's happening - some nucleophilic halogenation?
> > >
> > > wolfgang
> > >
> > >
> > > [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> > > -----
> > > Dr. Wolfgang Schechinger
> > > Lab N233 (c/o Dr. Steve Roffler)
> > > Institute of Biomedical Sciences
> > > Academia Sinica
> > > 128 Yen-Chio Yuan Rd. Sec.2
> > > Taipei 115
> > > Taiwan R.O.C.
> > > Tel +886-2-2789-9152
> > > Fax +886-2-2782-9142
> > > Mobile +886-925-136893
> > > wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
> > > -----
> > >
> > > ---
> >
> >
> >
> >
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> -----
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
> 128 Yen-Chio Yuan Rd. Sec.2
> Taipei 115
> Taiwan R.O.C.
> Tel +886-2-2789-9152
> Fax +886-2-2782-9142
> Mobile +886-925-136893
> wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
> -----
>
> ---
>




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