Gel storage before immunoblotting

clarosa clarosa at biocomp.unl.edu
Fri Apr 27 10:29:34 EST 2001


storage of gel overnight is possible, for large proteins..small proteins will
diffuse on unfixed gels.. never did it for weeks!



Choe Woo-seok wrote:

> After SDS-PAGE, can the gel be stored before electroblotting or is it always
> better to
> be transfered immediately.
> In case, Gel storage is unavoidable due to lack of nitrocellulose membrane,
> what will
> be the best condition for stroage?
> Many thanks for any input.
>
> -------------------------------------------
>  Woo-seok Choe
>  Dept. of Chemical Engineering
>  University of Cambridge
>  Pembroke Street
>  Cambridge CB2 3RA, UK
> -------------------------------------------
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>               +44 01223 334 786 (Office)
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> -------------------------------------------




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