RPA with a DNA probe

Stefi~ xxxgifalone at tin.it
Sat Apr 28 15:22:23 EST 2001


Nick Theodorakis <nicholas_theodorakis at urmc.rochester.edu> wrote in message
> That's called a nuclease S1 protection, and it actually predates RPA by
some
> time. Standard mol. biol. technique books (Molecular Cloning, Current
> Techniques, etc.) should have a protocol, and now that you know what it's
called
> you know what to look for.
>
> If you need a protocol, I can e-mail you one.

Thx so much. I'll search a protocol for S1 nuclease protection and if I
don't find anything, I'll ask you for it.
With "denaturing" do u mean "keeping strands without secondary structure" or
""separating strands"?
Thanx!
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911





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