RPA with a DNA probe
xxxgifalone at tin.it
Sat Apr 28 15:22:23 EST 2001
Nick Theodorakis <nicholas_theodorakis at urmc.rochester.edu> wrote in message
> That's called a nuclease S1 protection, and it actually predates RPA by
> time. Standard mol. biol. technique books (Molecular Cloning, Current
> Techniques, etc.) should have a protocol, and now that you know what it's
> you know what to look for.
> If you need a protocol, I can e-mail you one.
Thx so much. I'll search a protocol for S1 nuclease protection and if I
don't find anything, I'll ask you for it.
With "denaturing" do u mean "keeping strands without secondary structure" or
gifalone at tin.it
More information about the Methods