RPA with a DNA probe

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Sun Apr 29 20:45:58 EST 2001


In <9cf886$hmg$1 at fe1.cs.interbusiness.it>, Stefi~ wrote:

[...]

>
>Thx so much. I'll search a protocol for S1 nuclease protection and if I
>don't find anything, I'll ask you for it.
>With "denaturing" do u mean "keeping strands without secondary structure" or
>""separating strands"?

Both happen. But for nuclease protection purposes, most people are thinking
about the second phenomenon. 

Nick



-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu




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