affect of label at PCR reaktion??

Tamas Rujan tamas.rujan at epigenomics.com
Mon Apr 30 07:59:43 EST 2001


Hi there!

Does anybody know something about the following effect:
you have two sets of primer pairs with identical sequences but one time
the forward primer is labeled with cy5 and one time the reverse primer
is labeled. The conditions of the the two PCR reactions are the same. 
Results: when the forward primer is labeled, the reation is complete and
you get one nice band on the agarose gel. When the reverse primer is
labeled, there is no PCR product.

I would be glad to hear any kind of comment!

-- 
Tamás Rujan	 Staff Scientist




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