ricky_boernke at gmx.net
Wed Aug 1 02:06:39 EST 2001
Wolfgang Schechinger wrote:
> Hi all,
> what's the reason for using MgSO4 (instead of sodium
> chloride as in LB) in PSI broth? Is it esential for making
> competent cells?
We have used PSI for making competent cells following the Hanahan
protocol for years. However, we found that using LB makes no
difference. Thus we don't use PSI anymore.
> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
> 128 Yen-Chio Yuan Rd. Sec.2
> Taipei 115
> Taiwan R.O.C.
> Tel +886-2-2789-9152
> Fax +886-2-2782-9142
> Mobile +886-925-136893
> e mail wolfsc at ibms dot sinica dot edu dot tw
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