PSI broth

[Ricky Boernke]

Wolfgang Schechinger wrote:
> 
> Hi all,
> 
> what's the reason for using MgSO4 (instead of sodium
> chloride as in LB) in PSI broth? Is it esential for making
> competent cells?
> 
> Wo
We have used PSI for making competent cells following the Hanahan
protocol for years. However, we found that using LB makes no
difference. Thus we don't use PSI anymore.
Ricky



> [tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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> Dr. Wolfgang Schechinger
> Lab N233 (c/o Dr. Steve Roffler)
> Institute of Biomedical Sciences
> Academia Sinica
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