Pseudomonas and pH

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Aug 1 07:18:30 EST 2001


Thanks Sergion: yes, I think P aeruginosa is a resistant bug too.  I am
going to test it out under various challenging conditions.  But I think it
can take it!

Regards, Mike.

On Wed, 1 Aug 2001, Sergio wrote:

> I work on P. putida and in my opinion it's a very resistent bug... if you don't
> get an answer here a good and fast experiment to check the viability of P.
> aureginosa in low pH medium is just to resuspend the cells in your buffer and
> counting the cfu at different times.
> Sergio
>
> Michael Witty wrote:
>
> > Dear All,
> >         my aim is to pull down Pseudomonas aeruginosa using an antibody to
> > an outer membrane protein and protein A Sepharose beads.  Then I want to
> > disrupt the attachement to the beads and plate the cells on LB plates.  I
> > know that glycine buffer pH3.0 will separate IgG and protein A, BUT will
> > Pseudomonas aeruginosa survive this?
> >
> > Is this a good or a dumb idea?  Regards, Mike.
>
>




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