Cloning of cDNA amplification products from silver

Anoopkumar Thekkuveettil t_anoop at satyam.net.in
Fri Aug 3 22:31:57 EST 2001


Dear Colette,

You can scrap the band and incubate in 2X PCR buffer (50ul) at 50 C for 1
hour (you can rinse the gel fragments in 2XPCR buffer at room temperature
before adding fesh 2X PCR buffer and incubating at 50C). Spin doun and take
supenatent and PCR amplify/clone. This work well with us both in radioactive
gels and silver gels.

Anoop 


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