T4 ligase question

Wolfgang Schechinger wolfsc at ibms.sinica.edu.tw
Sun Aug 5 21:18:48 EST 2001


Hi all, 

what actually happens, when there's no ATP left in the  
reaction? will ligase start eating up DNA and act as a DNAse 
(considering the ligation reaction being a real chemical 
reaction DNA1 + DNA2 + ATP <=> ligated_DNA + ADP + P) ?

Wo
[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw
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