T4 ligase question

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Aug 6 00:03:11 EST 2001


wolfsc at ibms.sinica.edu.tw ("Wolfgang Schechinger") wrote:
>Hi all, 
>
>what actually happens, when there's no ATP left in the  
>reaction? will ligase start eating up DNA and act as a DNAse 
>(considering the ligation reaction being a real chemical 
>reaction DNA1 + DNA2 + ATP <=> ligated_DNA + ADP + P) ?

T4 ligase, AFAIK, does not have nuclease activity. If so, 
absense of ATP would mean no ligation without any 
irreversible changes.

        - Dima




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