how-to-count-cells

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Wed Aug 8 01:10:26 EST 2001


Hi Arturo,

one rather easy way would be to determine total protein mass/well with the
sulforhodamineB assay (Monks, A. et al. (1991). J Natl Cancer Inst 83,
757-66. and Skehan, P.et al. (1990). J Natl Cancer Inst 82, 1107-12.). It's
cheap, simple, reproducible, but kills your cells and it's not so fast
(drying time)... otherwise a microplate reader might pick up the turbidity.

Bruno

"J. Arturo García-Horsman" <arturo.garcia-horsman at uku.fi> wrote in message
news:3B700261.EDBA0549 at uku.fi...
> Hello all,
>
> I was wondering if anybody knows a method to determine the number cells
> growing in a 96 well plate. Something like measuring the turbidity
> (absorbance) or refringence, other than actually counting them. I don't
> have access to a cell counter, and I don't want to spend hours counting
> them below the microscope well by well.
>
> Any help would be appreciated.
>
> Cheers,
>
> Arturo.
> _______________________________________________________________
>
> Arturo Garcia-Horsman, Ph.D.
> Research Specialist
> Department of Pharmacology and Toxicology
> University of Kuopio
> Canthia, Harjulantie 1 A, 1. krs.
> 70210 Kuopio, Finland
> Tel: +358-017-162422
> Fax: +358-017-162424
> E-mail: Arturo.Garcia-Horsman at uku.fi
>
>
>





More information about the Methods mailing list