wood extraction of DNA

Dr. Klaus Eimert eimert at web.de
Thu Aug 9 02:31:47 EST 2001


dbell at qnis.net (dbell) wrote in news:01C11FED.C1DD4AA0.dbell at qnis.net:

> I am not quite sure what you are looking for but I hope this may help:
> When I was having problems with interfering substances from leaf
> extracts, I tried doing a basic STAB extraction on cambial tissue.  I
> would take a cutting, then peel back the bark layers, then scrape out
> the green cambial tissue with a scalpel or even a sharp potato peeler
> and use this to extract DNA from.  worked great, but it was time
> consuming. 
> 
> Good luck
> 
> Deanne Bell
> USDA Agricultural Research Service
> 2021 South Peach Avenue
> Fresno, CA 93727
> (559) 453-3170
> (559) 453-3088 fax
> 
> On Wednesday, August 08, 2001 2:02 AM, Francesca De Amicis 
> [SMTP:francesca.deamicis at dpvta.uniud.it] wrote:
>: I would like to know if there is a specific protocol for DNA
>: extraction from wood.
>: Thank you
>: Francesca De Amicis
>: 
> 
> ---
> 

Hi,

you might check the paper by Wolf et al. 1999 Austr. J. of Grape and Wine 
Res. 5: 34-38.  
I can send you the protocol if you cannot access the paper.
Regards,

Klaus

-- 
---------------------------------------------------------------------
Dr. Klaus Eimert
Dept. of Botany
State Research Institute Geisenheim                 
Von-Lade-Str. 1                                     
D-65366 Geisenheim                                  
Germany

Phone:  + 49 6722 502 469
Fax:       + 49 6722 502 460
E-Mail: eimert at web.de




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