dam- and dcm- e.coli cells

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Thu Aug 9 04:30:26 EST 2001

In article <Pine.LNX.4.10.10108081234130.17443-100000 at kelvin.ncl.res.in>
, the eminent T V Naga Rama Chander at BIOSCI/MRC Human Genome Mapping
Project Resource Centre wrote
>as understood e.coli jm109 is dcm- aut not dam-.


JM109 is both dcm+ dam+ and therefore BclI will not cut.

What you need is JM110 or GM2163, both available FOC (with any normal
order) from NEB.

It might look like I'm doing nothing, but at the cellular level I'm really
quite busy.

Duncan Clark
GeneSys Ltd.
Tel. +44 (0) 1252376288
FAX +44 (0) 8701640382

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