dam- and dcm- e.coli cells

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Thu Aug 9 04:30:26 EST 2001


In article <Pine.LNX.4.10.10108081234130.17443-100000 at kelvin.ncl.res.in>
, the eminent T V Naga Rama Chander at BIOSCI/MRC Human Genome Mapping
Project Resource Centre wrote
>
>as understood e.coli jm109 is dcm- aut not dam-.

Wrong.

JM109 is both dcm+ dam+ and therefore BclI will not cut.

What you need is JM110 or GM2163, both available FOC (with any normal
order) from NEB.

Duncan 
-- 
It might look like I'm doing nothing, but at the cellular level I'm really
quite busy.

Duncan Clark
GeneSys Ltd.
Tel. +44 (0) 1252376288
FAX +44 (0) 8701640382




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