renaud leonard renaudleo at
Thu Aug 9 08:01:53 EST 2001

Do you have tryed to do a preclearing with an other antibody of the same
ecotype but not diriged against your epitope ? it's a usefull technic to
reduce the background. You could do an affinity column with your antibody to
keep more material. Are you ensured that you are working in the good
denaturing conditions. I explain : is your antibody espressed agains a
native or a denaturated protein and dou you work in the appropriate
Hope to help you.


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