SDS debate

Malay curiouser at ccmb.ap.nic.in
Sat Aug 11 15:51:44 EST 2001


>
> "... SDS is an effective protein denaturant. It binds to all proteins
> qualitatively the same, at about 1.4 g per gram of amino acid. There is
one
> negative charge on each SDS molecule. The resulting charge density due to
all
> the SDS in the SDS-protein complex more-or-less overwhelms variations in
the
> charges of different protein molecules. ... Charles Tanford and others
have
> studied the structure of SDS-protein complexes. Hydrodynamically, they
appear to
> be prolate ellipsoids or rods with a constant diameter of about 18
angstroms and
> a length that is linear function of the molecule. ..."
>

The binding of SDS to protein is very well know. Omm... that requires a
little research to see whether Tanford had boiled the protein with SDS
before checking structure. Will look into it.

Regards,

Malay
Malay Kumar Basu
Centre for Cellular and Molecular Biology
Hyderabad 500007
I N D I A

Fax: (00-91)40-7171195
Phone: (00-91)40-7172241
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Tact: making a point without making an enemy.
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curiouser at ccmb.ap.nic.in



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