mw132 at mole.bio.cam.ac.uk
Sun Aug 12 07:16:46 EST 2001
Dear Malay and Emir,
thanks for your emails.
> What I really want to know is ( a very vital point):
> "Can SDS per se (without boiling) can denature a soluble protein?"
I think the answer is yes. For membrane proteins the advise is often
"don't boil the sample". Though I think trying it both ways is the best
way. This is because membrane proteins often aggregate when heated and
fail to enter the gel.
> ...There was even paper that most of the proteins takes a helical
> conformation in SDS. I can dig out the reference if anyone wants it
Yes please. I would appreciate knowing this reference. Regards, Mike.
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