mw132 at mole.bio.cam.ac.uk
Mon Aug 13 06:48:57 EST 2001
On Mon, 13 Aug 2001, Dr. Duncan Clark wrote:
> In article <9l3dh9$l1o$1 at news.rt66.com>, the eminent James Park at
> Rt66.COM, New Mexico's #1 ISP wrote
> >I am wondering if there are proteases or proteinases which can survive at
> >higher temperature (80oC).
> >The purpose is to digest tough proteins/glycoproteins at 80oC or 900C.
> Umpteen from hyperthermophilic archaea i.e. proteases that survive 100C
> plus. Problem is that I doubt any are commercially available. Best to
> search Pubmed and contact authors direct.
. . . interestingly one of my collegues once worked with thermophilic
bacterial proteins, hoping they would be heat resistant - most of them
were no more heat resistant than proteins from mesophillic bacteria (or is
this just lab gossip). Regards, Mike.
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