alkaline phosphatase...

Wolfgang Schechinger wolfsc at
Mon Aug 13 08:36:58 EST 2001

Hi all, 

Here is a debate going on if it is allowed (seems to some sort 
of dogma) to add SAP (or CIP) directly to a RE digest of a 
vector or better phosphatasing it in a separate step after 
purification of the digest. My opinion (and as I do it) is just add 
all to the mix and go, but the reason for the debate is how to 
minimize vector background when constructing a library.

(digest is NgoMIV + XhoI to cut vector, BamHI to kill old insert 
in NEB Buffer IV and SAP)

[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw


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