heat-resitant proteases?

James Park jpark100 at hotmail.com
Mon Aug 13 10:39:00 EST 2001

Dear Kresten,

I thought about subtilisin which is included in some laundry detergents.
I have no idea how stabile subtilisin is at 70C.
Could you please describe the buffer which you used for thermolysin?
I might try thermolysion.



"kresten" <no-sp at m.org> wrote in message
news:9l7ttl$jn2$1 at news.net.uni-c.dk...
> I have used thermolysin with success at 70 degrees. Among the several
> controls I had, one included adding a (partially) unfolded protein after
> incubating thermolysin for 30 min. The enzyme was still active.
> There are many references on the use of thermolysin.
> Kresten
> "James Park" <jpark100 at hotmail.com> wrote in message
> news:9l3dh9$l1o$1 at news.rt66.com...
> > I am wondering if there are proteases or proteinases which can survive
> > higher temperature (80oC).
> > The purpose is to digest tough proteins/glycoproteins at 80oC or 900C.
> > James.
> >
> >

More information about the Methods mailing list