silly? phenol question
Richard P. Grant
rpg14 at yahoo.co.uk.invalid
Tue Aug 14 07:26:25 EST 2001
<Pine.LNX.4.21.0108141515080.23601-100000 at biochem.iisc.ernet.in>,
skg at biochem.iisc.ernet.in ("SURESH KUMAR K.G.") wrote:
> When it goes off, it turns red, but again the transition point is
> difficult to tell. If its red even with HQ, better discard it.
I was, in fact, making the point that the yellow->red transition is
difficult to see. I've not used HQ since, oh, 1992, being able to
resolve the interface with most tubes in use.
tip: Store phenol (10 ml aliquots) under tris at -20. To make PCI, add
10 ml CHCl3:IAA and mix. Store *that* at -20, too. Doesn't go off, and
the small aliquots reduce the burn/death hazard. (100 ml phenol down a
leg is *not* pretty).
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk http://www2.mrc-lmb.cam.ac.uk/personal/rpg/
-- Hiroshima '45 - Chernobyl '86 - Windows 2000 --
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