alkaline phosphatase...

[Bryan Maloney]

wolfsc at ibms.sinica.edu.tw ("Wolfgang Schechinger") wrote in 
news:3B7849F4.4158.7D1C5F at localhost:

> Hi all, 
> 
> Here is a debate going on if it is allowed (seems to some sort 
> of dogma) to add SAP (or CIP) directly to a RE digest of a 
> vector or better phosphatasing it in a separate step after 
> purification of the digest. My opinion (and as I do it) is just add 
> all to the mix and go, but the reason for the debate is how to 
> minimize vector background when constructing a library.

I slam such digests with an overdose of CIP then gel purify the backbone.