bjm10 at cornell.edu
Tue Aug 14 07:42:45 EST 2001
wolfsc at ibms.sinica.edu.tw ("Wolfgang Schechinger") wrote in
news:3B7849F4.4158.7D1C5F at localhost:
> Hi all,
> Here is a debate going on if it is allowed (seems to some sort
> of dogma) to add SAP (or CIP) directly to a RE digest of a
> vector or better phosphatasing it in a separate step after
> purification of the digest. My opinion (and as I do it) is just add
> all to the mix and go, but the reason for the debate is how to
> minimize vector background when constructing a library.
I slam such digests with an overdose of CIP then gel purify the backbone.
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