Adaptors for join SalI to BglII

Peter Ashby p.r.ashby at dundee.MAPS.ac.uk
Tue Aug 14 08:51:34 EST 2001


In article <B795BD1D.1897%rozsa at umich.edu>, rozsa at umich.edu (frank) 
wrote:

> Question about using adaptors to convert restriction sites:
> I have a 4.5 kb insert flanked by SalI (5¹) and XbaI (3¹) , the vector has
> Bgl2 (5¹) and Xba (3¹).

If you partial fill Sal with C & T and partial fill BglII with G & A you 
get a compatible 2 base overhang which ligates very well in my 
experience. I used to do this routinely to get a promoter into a 
reporter in frame.

Peter

-- 
Peter Ashby
Wellcome Trust Biocentre
University of Dundee
Dundee, Scotland
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