How to design oligos based on variable peptide sequence?
khatipo at ZAPuchicago.edu
Tue Aug 14 11:41:13 EST 2001
I think you response is a bit out of point. I clearly asked for software. I
certainly understand that spreadsheet can do that, but it is a harder and
longer way. Time is really an issue for me right now, so I am looking for
"Bryan Maloney" <bjm10 at cornell.edu> wrote in message
news:9lb6ah$kqp$4 at news01.cit.cornell.edu...
> "Emir Khatipov" <khatipo at ZAPuchicago.edu> wrote in
> news:AVUd7.261$65.19645 at news.uchicago.edu:
> > Dear netters,
> > I want to construct a peptide library. I have a 10 peptide stretch in
> > which some aa residues have to be conserved, others should vary,
> > basically something like:
> > A-(V,I,L,M, or C)-K-(F, Y, or W)-P-(S, T, G, or D)-...etc.
> > Could you share your experience on how I should better design
> > degenerate oligonucleotide sequence based on peptide sequences like
> It may sound crude (indeed, it is crude), but I'd use a spreadsheet rather
> than track down a piece of specialized software. By the time I find the
> software and learn to use it, I'll have the spreadsheet work finished.
> "Cape Cod Salsa--somehow that's just not right."
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