How to design oligos based on variable peptide sequence?
dmicklem at cmgm.nospam.invalid
Tue Aug 14 16:20:29 EST 2001
In article <WDce7.333$65.25282 at news.uchicago.edu>, Emir Khatipov
<khatipo at ZAPuchicago.edu> wrote:
>If I will really have to go for spreadsheet-assisted design of oligos, I
>would appreciate if someone could send me or post here a table of genetic
>code based on degenerate nucleotide code (M for C or A, R for A or G, etc).
>Don't remember seeing such a thing anywhere.
Well, it shouldn't be too tough to work that out for yourself! Five
minutes with the New England Biolabs catalogue gives:
Arg CGN or AGR (but MGR will inclide Ser too!)
Leu CTN or TTR (but YTN will include Phe too!)
Ser TCN or AGY (but YSN will include all sorts of stuff)
although you might want to double check it!
D.R. Micklem, Time flies like an arrow...
Dept. of Anatomy, Fruit flies like a banana.
Cambridge University, Email:dmicklem at cmgm.stanford.edu
Cambridge, UK Phone: +44 (1223) 333776
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