P1 lysogeny: detection and curing

[Dr. Duncan Clark]

In article <F41bZhcoT5psudxkzad0000ce30 at hotmail.com>, the eminent Robert
Whittier at BIOSCI/MRC Human Genome Mapping Project Resource Centre
wrote
>Tip of the hat and thank-you to Dr. Duncan Clark for his apparently
>correct diagnosis and helpful suggestion.

Nice to know the old grey matter still works on occasions.

I have to admit I was slightly cheating due to knowing someone who once
had a post-doc project cloning 'unclonable' DNA i.e. repeats and the
only answer then was lower copy no., anything but a pBR derived plasmid
(she swears there is some sequence on those and it really plays havoc
with stabilising repeats) and an appropriate E.coli host found by trial
and error.

Duncan
-- 
Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK 

http://www.dstl.gov.uk/html/specialisations/index.htm