humanizing a gene

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Thu Aug 16 07:54:07 EST 2001


In article <Pine.SGI.4.33.0108161318470.4915564-100000 at mole.bio.cam.ac.u
k>, the eminent Michael Witty at University of Cambridge, England wrote
>BUT I am taking a Pseudomonas coding sequence and making all
>the rare codons into E. coli preferred codons.  What should I call this?

Not sure but beware of introducing secondary structure in the start
amino acids thereby making the sequence behave like a transcription
terminator.

Duncan
-- 
Homogeneous Fluorescent Reporting Systems for Real-Time Quantitative PCR: 

Optimisation, Probe Technology & Future Systems

4-5 September 2001
King Alfred's College, Winchester, UK 

http://www.dstl.gov.uk/html/specialisations/index.htm




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