humanizing a gene
Michael L. Sullivan
mlsulliv at facstaff.wisc.edu
Thu Aug 16 08:32:59 EST 2001
Depending on what you want to use the word for, I'd perhaps suggest the
phrase "optimizing for expression in E. coli". It is more words, but less
ambiguous and more easily understood I think.
BTW, this is coming from somebody whose graduate advisor didn't like
"verbifying nouns" or "nouning verbs", e.g., we don't "pH" a solution, we
"adjust the pH of" a solution!
> if I was taking a heterologous coding sequence and making all the
>rare codons into human preferred codons I would be humanizing the coding
>sequence. BUT I am taking a Pseudomonas coding sequence and making all
>the rare codons into E. coli preferred codons. What should I call this?
>Escherichizing? Colizing? Colonizing? Please help with my genetic
Michael L. Sullivan, Ph.D
U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706
(608) 264-5144 Phone
(608) 264-5147 Fax
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