calcium free footprinting

Dr. Karthikeyan Narayanan karthi at
Thu Aug 16 10:57:52 EST 2001

Dear all:
I have a calcium binding protein which shows a band shift with appropriate 
dsDNA. I 
wanted to get the specific binding sequence for this protein. The thing is, 
since it is a 
calcium binding protein even nM concentration of calcium alters the structure 
as seen by 
the CD analysis. I tried to use the DNaseI for footprinting, unfortunately, 
all the 
DNase were active only in the presence of calcium ions. Is there any other 
method I 
could try to get the binding sequence. Any help would be appreciated.

Karthikeyan Narayanan Ph.D.,	
Department of Oral Biology,
College of Dentistry,
801, S. Paulina st.,
Rm 437
University of Illinois at Chicago,
Il 60612
Tel: (312) 413-5375 (lab)
Fax: (312) 996-6044


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