calcium free footprinting

[Dr. Karthikeyan Narayanan]

Dear all:
I have a calcium binding protein which shows a band shift with appropriate 
dsDNA. I 
wanted to get the specific binding sequence for this protein. The thing is, 
since it is a 
calcium binding protein even nM concentration of calcium alters the structure 
as seen by 
the CD analysis. I tried to use the DNaseI for footprinting, unfortunately, 
all the 
DNase were active only in the presence of calcium ions. Is there any other 
method I 
could try to get the binding sequence. Any help would be appreciated.
Thanks
Karthi

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Karthikeyan Narayanan Ph.D.,	
Department of Oral Biology,
College of Dentistry,
801, S. Paulina st.,
Rm 437
University of Illinois at Chicago,
Chicago 
Il 60612
Tel: (312) 413-5375 (lab)
Fax: (312) 996-6044
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