Quantitative determination of Catalase protein?
Stewart P Johnson
Johns053 at mc.duke.edu
Thu Aug 16 15:58:24 EST 2001
An ancient assay was to measure the decrease in the amount of hydrogen
peroxide at 240 nm. If you want a detailed procedure, check out the
Worthington manual. There are some more modern methods using fluorometric
probes if you want to get fancy. Personally, I haven't done a catalase
assay in 25 years, but it is a simple enough assay that student labs do
some version of it.
Best of luck, Stewart
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