detection of small differences in dna fragment sizes

Nick Jacobsen nickjacobsen at yahoo.co.uk
Fri Aug 17 05:32:38 EST 2001


"Nicole Baumert" <nicole at mibi03.meb.uni-bonn.de> wrote in message news:<9lgian$s18$1 at f1node01.rhrz.uni-bonn.de>...
> Hallo!
> Does anyone know a good and reproducable method for the detection of small
> differences (1-10 basepairs) in dna fragment sizes? e.g. a special agarose
> and/ or a protocol.
> Thanks, Nicole.



Hi Nicole,

try using Metaphor agarose from Boehringer (i think its boehringer).
Basically you make a 2% metaphor/1% normal agarose gel or more if you
need higher resolution. You get really good resolution on small
differences.

cheers
Nick




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