Precipitation in PCR Product (PERT Assay)

Kimberly Brue kimberly.brue at aventis.com
Fri Aug 17 06:55:28 EST 2001


I am doing a coupled product enhanced reverse transcriptase assay (to
detect for rt activity in virus-amplified avian cell culture
supernatant). My PCR products contained a whitish precipitate (which
went back into solution with pipetting), and I had very faint bands on
the gel. Can anyone offer any explanation for the precipitate?




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