fluorescence question

kresten no-sp at m.org
Mon Aug 20 03:45:19 EST 2001

What about the excitation wavelength. Was that the same as that reported to
give emission maximum at 570 nm?


"Balaji Kannan" <balajik at newton.berkeley.edu> wrote in message
news:9lilbd$iet$1 at agate.berkeley.edu...
> hi all
>     i was using a cyanin dye called 5' Cy3 that is supposed to emit max at
> 570 nm.. when i analysed my sample to check if it contained the cyanin
> i got a big emission peak at 535 nm and a much smaller one at 554 nm. the
> whole emission spectrum from this point onwards is flat. i am very sure
> there is no other fluorescent material in my sample. my question now is-
> these peaks convincing evidence that my sample has the cyanin dye? i mean,
> is it okay for a dye that emits max at 570 to give some peak at 535/555
>     thanks
>     balajik

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