enzyme mismatch cleavage mutation detection

Bruno Cenni bruno.removethis.cenni at removethis.insel.ch
Tue Aug 21 06:31:59 EST 2001


hi all,

has anyone used enyme mismatch cleavage for detection of mutations?
Pharmacia/Amersham have the passport kit that is based on T4 endonuclease
VII. Anyone any experiences or suggestions for alternative techniques (we
want to screen cDNA pieces in the range of 1-5 kb for unknown mutations).

TIA

bruno





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