problems with primer dimer!

[Wolfgang Schechinger]

Babett, 

Probably happens when freeze thawing primers for many 
times.
Try melting the primers 10 min or more at 94 degC, then put 
the vial into ice water. This procedure should break up all 
primer primer interactions.
If not, order new ones and aliquot them after dilution into 
convenient fractions of e.f. 25 ul each. That's probably cheaper 
than spending days in finding the true cause. Where do you 
get your oligos from?

Wolfgang

> Hope somebody can help me.
> We investigated gene expression using PCR without any problems.
> Since last week, we suddenly have a lot of primer dimers and
> problems with the right size band. How can we solve the problem
> with the excess of primer dimers??
> 
> Thanks in advance
> 
> Andreas
> 
> 
> 
> 


[tiss mezzage wahs broduceRd using TYPO GENERATOR zoffwer]
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Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
128 Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2789-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
e mail wolfsc at ibms dot sinica dot edu dot tw
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