problems with primer dimer!
babett.bartling at medizin.uni-halle.de
Mon Aug 27 09:29:39 EST 2001
the primers were from Gibco.
We will try to melt the primers and repeat the PCR.
Wolfgang Schechinger schrieb:
> Probably happens when freeze thawing primers for many
> Try melting the primers 10 min or more at 94 degC, then put
> the vial into ice water. This procedure should break up all
> primer primer interactions.
> If not, order new ones and aliquot them after dilution into
> convenient fractions of e.f. 25 ul each. That's probably cheaper
> than spending days in finding the true cause. Where do you
> get your oligos from?
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