problems with primer dimer!

Babett Bartling babett.bartling at medizin.uni-halle.de
Mon Aug 27 09:29:39 EST 2001


Wolfgang,
the primers were from Gibco.
We will try to melt the primers and repeat the PCR.

Babett&Andreas



Wolfgang Schechinger schrieb:

> Babett,
>
> Probably happens when freeze thawing primers for many
> times.
> Try melting the primers 10 min or more at 94 degC, then put
> the vial into ice water. This procedure should break up all
> primer primer interactions.
> If not, order new ones and aliquot them after dilution into
> convenient fractions of e.f. 25 ul each. That's probably cheaper
> than spending days in finding the true cause. Where do you
> get your oligos from?
>
> Wolfgang
>




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